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tlr7 agonist cl 097  (InvivoGen)


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    Structured Review

    InvivoGen tlr7 agonist cl 097
    Tlr7 Agonist Cl 097, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tlr7+agonist+cl+097/CL097/10__1172_slash_jci189566-234-40-43
    Average 95 stars, based on 289 article reviews
    tlr7 agonist cl 097 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Confocal Microscopy:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Purification:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Software:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Comparison:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Activation Assay:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Labeling:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Flow Cytometry:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    MANN-WHITNEY:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Mutagenesis:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Functional Assay:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Generated:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Protein-Protein interactions:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Derivative Assay:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    In Vitro:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Cell Culture:

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37°C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50μM of 2- mercaptoethanol) and then stimulated at ten million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen) as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.

    Article Title: Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
    Article Snippet: as indicated in the figure legends) were isolated as per flow cytometry methods in Stem Cell Buffer (PBS with 2% Fetalplex serum and 1mM EDTA). .. Cells were warmed at 37C for 45 minutes in media (RPMI 1640 with 10% Fetalplex, Glutamax, Penicillin/Streptomycin, HEPES and 50M of 2-mercaptoethanol) and then stimulated at 10 million cells/ml with CpG DNA (CpG ODN 1826, Hokkaido System Science) and TLR7 agonist CL_097 (Invivogen), as indicated in the figure legends. .. Cells were fixed with 1.5% PFA for 15 minutes at room temperature and permeabilized in FACS-staining buffer containing 0.1% Triton X-100.



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    InvivoGen tlr7 agonist cl 097
    Tlr7 Agonist Cl 097, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tlr7+agonist+cl+097/CL097/10__1172_slash_jci189566-234-40-43
    Average 95 stars, based on 1 article reviews
    tlr7 agonist cl 097 - by Bioz Stars, 2026-09
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